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pcDNA mouse NHERF-1 (1-325)
(Plasmid #32706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Hygro(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
    NHERF-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    Deleted last 30 AA putative Ezrin binding site
  • GenBank ID
    U74079
  • Entrez Gene
    Nherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
  • Promoter CMV
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site v=Hind3 i=Xho1 filled 7 blunted (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA mouse NHERF-1 (1-325) was a gift from Edward Weinman (Addgene plasmid # 32706 ; http://n2t.net/addgene:32706 ; RRID:Addgene_32706)

  • For your References section:

    Ezrin binding domain-deficient NHERF attenuates cAMP-mediated inhibition of Na(+)/H(+) exchange in OK cells. Weinman EJ, Steplock D, Wade JB, Shenolikar S. Am J Physiol Renal Physiol. 2001 Aug;281(2):F374-80. 10.1152/ajprenal.2001.281.2.F374 PubMed 11457730
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