pcDNA3.1(+)-GRP78/BiP
(Plasmid #32701)

• Depositing Lab: Richard C. Austin
• Publication: Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GRP78/BiP
• Species: H. sapiens (human)
• Insert Size (bp): 1950
• Entrez Gene: HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XHOI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The D238G point mutation found in the Addgene sequence is a known variation in human GRP78 that does not alter its chaperone or ATPase activities.