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pAXEF-Rap1-E63(GTP)
(Plasmid #32698)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAXEF-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    P3-episome expressing bacteria to activate the tet resistance
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RAP1
  • Species
    H. sapiens (human)
  • Mutation
    E63: Consitutively GTP-bound
  • Entrez Gene
    RAP1A (a.k.a. C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21)
  • Promoter EF promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer EF-1a-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Q63 changed to E to give phenotype

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAXEF-Rap1-E63(GTP) was a gift from Vicki Boussiotis (Addgene plasmid # 32698 ; http://n2t.net/addgene:32698 ; RRID:Addgene_32698)

  • For your References section:

    RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Lafuente EM, van Puijenbroek AA, Krause M, Carman CV, Freeman GJ, Berezovskaya A, Constantine E, Springer TA, Gertler FB, Boussiotis VA. Dev Cell. 2004 Oct;7(4):585-95. 10.1016/j.devcel.2004.07.021 PubMed 15469846
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