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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4754
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGRK4
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Alt nameGRK4alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)1800
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Entrez GeneGRK4 (a.k.a. GPRK2L, GPRK4, GRK4a, IT11)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the longest splice variant of GRKa. The insert does not contain the 3'UTR.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-hGRK4 was a gift from Robert Lefkowitz (Addgene plasmid # 32690 ; http://n2t.net/addgene:32690 ; RRID:Addgene_32690) -
For your References section:
Characterization of the G protein-coupled receptor kinase GRK4. Identification of four splice variants. Premont RT, Macrae AD, Stoffel RH, Chung N, Pitcher JA, Ambrose C, Inglese J, MacDonald ME, Lefkowitz RJ. J Biol Chem. 1996 Mar 15;271(11):6403-10. 10.1074/jbc.271.11.6403 PubMed 8626439