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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGRK3
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Alt nameG protein-coupled receptor kinase 3
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SpeciesB. taurus (bovine)
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Entrez GeneGRK3 (a.k.a. ADRBK2, BARK2)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (not destroyed)
- 3′ cloning site None (not destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-GRK3 was a gift from Robert Lefkowitz (Addgene plasmid # 32689 ; http://n2t.net/addgene:32689 ; RRID:Addgene_32689) -
For your References section:
Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies. Pitcher JA, Fredericks ZL, Stone WC, Premont RT, Stoffel RH, Koch WJ, Lefkowitz RJ. J Biol Chem. 1996 Oct 4. 271(40):24907-13. 10.1074/jbc.271.40.24907 PubMed 8798768