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Addgene

pENTR.hU6hH1
(Plasmid #32686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 2720
  • Modifications to backbone
    hU6hH1 dual shRNA expression cassette
  • Vector type
    Mammalian Expression, RNAi
  • Promoter hU6hH1
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR.hU6hH1 was a gift from Ming-Sound Tsao (Addgene plasmid # 32686 ; http://n2t.net/addgene:32686 ; RRID:Addgene_32686)

  • For your References section:

    Modified gateway system for double shRNA expression and Cre/lox based gene expression. Radulovich N, Leung L, Tsao MS. BMC Biotechnol. 2011 Mar 22;11:24. 10.1186/1472-6750-11-24 PubMed 21418658
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