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Addgene

pLKO.DEST.hygro
(Plasmid #32685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone size (bp) 7032
  • Modifications to backbone
    Gateway destination cassette
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.DEST.hygro was a gift from Ming-Sound Tsao (Addgene plasmid # 32685 ; http://n2t.net/addgene:32685 ; RRID:Addgene_32685)

  • For your References section:

    Modified gateway system for double shRNA expression and Cre/lox based gene expression. Radulovich N, Leung L, Tsao MS. BMC Biotechnol. 2011 Mar 22;11:24. 10.1186/1472-6750-11-24 PubMed 21418658
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