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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACAGW-ChR2-Venus-AAV
- Backbone size (bp) 4427
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Modifications to backboneRemoved CAGW-ChR2-Venus insert and replaced with Gateway aatR1-aatR2 cassette
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Vector typeAAV ; aav
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bypACAGW-ChR2-Venus-AAV originally obtained from Karel Svoboda via Addgene (plasmid 20071)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Gateway was a gift from Matthew Nolan (Addgene plasmid # 32671 ; http://n2t.net/addgene:32671 ; RRID:Addgene_32671) -
For your References section:
A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812
Map uploaded by the depositor.
