pAAV-Gateway
(Plasmid #32671)

• Purpose: (Empty Backbone)
• Depositing Lab: Matthew Nolan
• Publication: White et al Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.

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• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
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  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
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Backbone

• Vector backbone: pACAGW-ChR2-Venus-AAV
• Backbone size (bp): 4427
• Modifications to backbone: Removed CAGW-ChR2-Venus insert and replaced with Gateway aatR1-aatR2 cassette
• Vector type: AAV ; aav

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pACAGW-ChR2-Venus-AAV originally obtained from Karel Svoboda via Addgene (plasmid 20071)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry