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Addgene

pISceI Dusp6:D2EGFP
(Plasmid #32667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSce1d2EGFP
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Zebrafish Transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dusp6 promoter
  • Alt name
    Dusp6
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    10000
  • Entrez Gene
    dusp6 (a.k.a. Mkp3, wu:fd99d09)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISceI Dusp6:D2EGFP was a gift from Michael Tsang (Addgene plasmid # 32667 ; http://n2t.net/addgene:32667 ; RRID:Addgene_32667)

  • For your References section:

    Generation of FGF reporter transgenic zebrafish and their utility in chemical screens. Molina GA, Watkins SC, Tsang M. BMC Dev Biol. 2007 Jun 6;7:62. 10.1186/1471-213X-7-62 PubMed 17553162
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