-
PurposeRabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32646 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSADdeltaG
-
Backbone manufacturerAddgene plasmid 32634
- Backbone size w/o insert (bp) 15428
-
Vector typeMammalian Expression ; Rabies virus
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameChR2
-
Insert Size (bp)1600
- Promoter CMV
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSADdeltaG-ChR2-mCherry was a gift from Edward Callaway (Addgene plasmid # 32646 ; http://n2t.net/addgene:32646 ; RRID:Addgene_32646) -
For your References section:
New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879
Map uploaded by the depositor.
