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Addgene

pSADdeltaG-GCaMP3-DsRedX
(Plasmid #32645)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 32645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSADdeltaG
  • Backbone manufacturer
    Addgene plasmid 32634
  • Backbone size w/o insert (bp) 15428
  • Vector type
    Mammalian Expression ; Rabies virus
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GCaMP3
  • Insert Size (bp)
    1400
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sbf1 (not destroyed)
  • 3′ cloning site PacII (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    DsRedX
  • Insert Size (bp)
    700

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADdeltaG-GCaMP3-DsRedX was a gift from Edward Callaway (Addgene plasmid # 32645 ; http://n2t.net/addgene:32645 ; RRID:Addgene_32645)

  • For your References section:

    New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879
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