pSADdeltaG-GFP-mCherry
(Plasmid #32643)

• Depositing Lab: Edward Callaway
• Publication: Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Backbone

• Vector backbone: pSADdeltaG
• Backbone manufacturer: Addgene plasmid 32634
• Backbone size w/o insert (bp): 15428
• Vector type: Mammalian Expression ; Rabies virus
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP
• Insert Size (bp): 700
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Gene/Insert 2

• Gene/Insert name: mCherry
• Insert Size (bp): 700

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate rabies virus vectors which express two genes in different segments, we
inserted the rabies transcription stop and start signals followed by mCherry ORF between
the GFP stop codon and the original transcription stop signal of GFP.
5’-TAActgcagCATGAAAAAAActAACACCCCTCCactagtcgccaccATG-3’ The underlined sequences represent the transcription stop and start signals and the stop codon of GFP and the start codon of mCherry are shown at the 5’ and 3’ ends, respectively. Then, the partial cDNA was ligated into pcDNA-SADdeltaG-GFP, resulting in pcDNASADdeltaG-GFP-mCherry.