pGEMTEZ-Kir2.1
(Plasmid #32641)

• Depositing Labs: Richard Axel, Joseph Gogos, C. Ron Yu
• Publication: Yu et al Neuron. 2004 May 27;42(4):553-66.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kir2.1
• Species: M. musculus (mouse)
• Insert Size (bp): 1287

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.