pGEMTEZ-TeTxLC
(Plasmid #32640)

• Depositing Labs: Richard Axel, Joseph Gogos, C. Ron Yu
• Publication: Yu et al Neuron. 2004 May 27;42(4):553-66.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetanus toxin light chain
• Alt name: TeTxLC

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results identified single nucleotide insertions at bp# 2955/2956 and 4357/4358, as well as a 3 nucleotide insertion at bp# 4344/4345, when compared to the author's sequence. The insertions are outside of the coding region and should not be a concern for the intended purpose of the plasmid