Skip to main content
Addgene

pSADdeltaG-mCherry-Myc
(Plasmid #32637)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 32637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSADdeltaG
  • Backbone manufacturer
    Addgene plasmid 32634
  • Backbone size w/o insert (bp) 15428
  • Vector type
    Mammalian Expression ; Rabies virus
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    1000
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xMyc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The genes which replaced GFP in the pcDNA-SADdeltaG-GFP plasmid were amplified by
PCR using plasmids encoding mCherry and primer pairs with BamHI and NheI/NotI
sequences at the ends.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADdeltaG-mCherry-Myc was a gift from Edward Callaway (Addgene plasmid # 32637 ; http://n2t.net/addgene:32637 ; RRID:Addgene_32637)

  • For your References section:

    New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879
Related items:
Commonly requested with: