pSADdeltaG-F3
(Plasmid #32634)

• Depositing Lab: Edward Callaway
• Publication: Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Backbone

• Vector backbone: pcDNA3.1/Zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B19N, B19P, B19M and B19L
• Species: Rabies virus
• Insert Size (bp): 10000
• Mutation: In frame insertion of Ser after aa10 in M (matrix protein).
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences.
The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.