pCAG:myr-mRFP1
(Plasmid #32604)

• Depositing Lab: Anna-Katerina Hadjantonakis
• Publication: Nowotschin et al Genesis. 2009 May;47(5):330-6.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4698
• Modifications to backbone: pCX::myr-RFP was generated by PCR of mRFP1 from pRSET-mRFP1. The resulting PCR product was cloned into a pCRII-TOPO (Invitrogen) and the insert excised with EcoRI and cloned into pCAGGS (Niwa et al., 1991).
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myr-mRFP1
• Species: jellyfish
• Promoter: pCAGGs

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GCC TCC TCC GAG GAC GTC ATC AAG G
• 3′ sequencing primer: CAA GC TTC GAA TTC TTA GGC GCC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12) https://www.ncbi.nlm.nih.gov/pubmed/12060735 .