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pCAG:mRFP1
(Plasmid #32600)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRFP1
  • Promoter pCAGGs

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg)
  • 3′ sequencing primer 3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mRFP1 coding sequence was amplified by PCR using
primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and
3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx
Polymerase (Invitrogen). The resulting product was
cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene
6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and
verify that it produced robust red fluorescence

More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG:mRFP1 was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32600 ; http://n2t.net/addgene:32600 ; RRID:Addgene_32600)

  • For your References section:

    Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. Long JZ, Lackan CS, Hadjantonakis AK. BMC Biotechnol. 2005 Jul 4;5:20. 10.1186/1472-6750-5-20 PubMed 15996270
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