pCAG:ECFP
(Plasmid
#32597)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameECFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer GFP-L (5'- TTG AAT TCG CCA CCA TGG TGA GC)
- 3′ sequencing primer GFP-R (5'- TTG AAT TCT TAC TTG TAC AGC TCG TCC) (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG:ECFP was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32597 ; http://n2t.net/addgene:32597 ; RRID:Addgene_32597) -
For your References section:
Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. Hadjantonakis AK, Macmaster S, Nagy A. BMC Biotechnol. 2002 Jun 11;2:11. 10.1186/1472-6750-2-11 PubMed 12079497
Map uploaded by the depositor.
