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Addgene

pCAG:EYFP
(Plasmid #32596)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EYFP
  • Promoter pCAGGs

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GFP-L (5'- TTG AAT TCG CCA CCA TGG TGA GC)
  • 3′ sequencing primer GFP-R (5'- TTG AAT TCT TAC TTG TAC AGC TCG TCC)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG:EYFP was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32596 ; http://n2t.net/addgene:32596 ; RRID:Addgene_32596)

  • For your References section:

    Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. Hadjantonakis AK, Macmaster S, Nagy A. BMC Biotechnol. 2002 Jun 11;2:11. 10.1186/1472-6750-2-11 PubMed 12079497
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