pENTR-L3-IRES-EGFP-L2
(Plasmid
#32591)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221 P3-P2
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Backbone manufacturerInvitrogen
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Vector typeGateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRES-EGFP
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymodified material is amplified from the material provided by Dr Patrick Martin, Gepitos Laboratory, Joint Research Unit CNRS/Universite de Nice Sophia Antipolis (UMR 6235)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
BMC Biotechnol. 2006 Jan 12;6:4.
Development of a new bicistronic retroviral vector with strong IRES activity.
Martin P, Albagli O, Poggi MC, Boulukos KE, Pognonec P.
Source
CNRS UMR6548, Parc Valrose, Université de Nice Sophia Antipolis, Nice, France. [email protected]
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-L3-IRES-EGFP-L2 was a gift from Matthew Nolan (Addgene plasmid # 32591 ; http://n2t.net/addgene:32591 ; RRID:Addgene_32591) -
For your References section:
A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812