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Addgene

pENTR-L3-IRES-EGFP-L2
(Plasmid #32591)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32591 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221 P3-P2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRES-EGFP

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    modified material is amplified from the material provided by Dr Patrick Martin, Gepitos Laboratory, Joint Research Unit CNRS/Universite de Nice Sophia Antipolis (UMR 6235)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BMC Biotechnol. 2006 Jan 12;6:4.
Development of a new bicistronic retroviral vector with strong IRES activity.
Martin P, Albagli O, Poggi MC, Boulukos KE, Pognonec P.
Source
CNRS UMR6548, Parc Valrose, Université de Nice Sophia Antipolis, Nice, France. [email protected]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-L3-IRES-EGFP-L2 was a gift from Matthew Nolan (Addgene plasmid # 32591 ; http://n2t.net/addgene:32591 ; RRID:Addgene_32591)
  • For your References section:

    A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812