pENTR-L1-SYN-R5
(Plasmid
#32582)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221 P1-P5r
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Backbone manufacturerInvitrogen
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Vector typeGateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynapsin I promoter
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySYN and ESYN sequences used in these plasmids were provided by Hiroyuki Hioki from the Graduate School of Medicine, Kyoto University, Japan
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924
Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters
H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-L1-SYN-R5 was a gift from Matthew Nolan (Addgene plasmid # 32582 ; http://n2t.net/addgene:32582 ; RRID:Addgene_32582) -
For your References section:
A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812