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Addgene

pENTR-L1-ESYN-R5
(Plasmid #32581)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32581 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR221 P1-P5r
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Synapsin Promoter
  • Species
    H. sapiens (human)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SYN and ESYN sequences used in these plasmids were provided by Hiroyuki Hioki from the Graduate School of Medicine, Kyoto University, Japan
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924

Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters

H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-L1-ESYN-R5 was a gift from Matthew Nolan (Addgene plasmid # 32581 ; http://n2t.net/addgene:32581 ; RRID:Addgene_32581)
  • For your References section:

    A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812