pENTR-L1-ESYN-L4
(Plasmid #32580)

• Depositing Lab: Matthew Nolan
• Publication: White et al Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.

Backbone

• Vector backbone: pDONR221 P1-P4
• Vector type: Gateway cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Synapsin Promoter
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SYN and ESYN sequences used in these plasmids were provided by Hiroyuki Hioki from the Graduate School of Medicine, Kyoto University, Japan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The attL1 site within this plasmid is missing the last 3 nucleotides. The depositor states that this discrepancy does not affect plasmid function.

Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924

Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters

H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko