pXcX-G4BS-3.6TPH-EGFP
(Plasmid
#32572)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXcX
- Backbone size w/o insert (bp) 7076
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP
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Speciesjellyfish
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Insert Size (bp)798
- Promoter 3.6TPH
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer atggtgagcaagggcgaggagctg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's quality control sequence shows A inserted relative to the author's sequence. There is no evidence that this insertion affects the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXcX-G4BS-3.6TPH-EGFP was a gift from Sergey Kasparov (Addgene plasmid # 32572 ; http://n2t.net/addgene:32572 ; RRID:Addgene_32572) -
For your References section:
Adenoviral vectors for highly selective gene expression in central serotonergic neurons reveal quantal characteristics of serotonin release in the rat brain. Benzekhroufa K, Liu B, Tang F, Teschemacher AG, Kasparov S. BMC Biotechnol. 2009 Mar 19;9:23. 10.1186/1472-6750-9-23 PubMed 19298646