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Addgene

pCMV myc DAF-12
(Plasmid #32566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV myc
  • Backbone size w/o insert (bp) 3791
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DAF-12
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2214
  • Entrez Gene
    daf-12 (a.k.a. CELE_F11A1.3)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc epitope (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer M13(-21)
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV myc DAF-12 was a gift from Al Fisher (Addgene plasmid # 32566 ; http://n2t.net/addgene:32566 ; RRID:Addgene_32566)

  • For your References section:

    DAF-12 Regulates a Connected Network of Genes to Ensure Robust Developmental Decisions. Hochbaum D, Zhang Y, Stuckenholz C, Labhart P, Alexiadis V, Martin R, Knolker HJ, Fisher AL. PLoS Genet. 2011 Jul;7(7):e1002179. Epub 2011 Jul 21. 10.1371/journal.pgen.1002179 PubMed 21814518
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