pBABe-HA-ER-IPpoI
(Plasmid #32565)

• Depositing Lab: Michael Kastan
• Publication: Berkovich et al Nat Cell Biol. 2007 Jun;9(6):683-90. Epub 2007 May 7.

Backbone

• Vector backbone: pBABE-Puro
• Backbone manufacturer: margenstern and L (1991) PMID Z194165
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: I-PpoI
• Species: Physarum polycephalum
• Insert Size (bp): 1514
• Mutation: none
• GenBank ID: PMD 17473858
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • ER (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: PBABE 5'

Resource Information

• Supplemental Documents:
  • Annotated map and sequence
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry