pUCBB-ntH6-eGFP
(Plasmid #32557)

• Depositing Lab: Claudia Schmidt-dannert
• Publication: Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.

Backbone

• Vector backbone: pUCBB
• Backbone size w/o insert (bp): 2455
• Modifications to backbone: Added N-terminal 6 His tag and Thrombin clevage site between the BglII site and NdeI site. Genes cloned with ndeI are inframe with the tag. In addition, a stop codon was placed inframe with the C-terminal XhoI site.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 719
• Entrez Gene: eGFP (a.k.a. pPRS3a_01)
• Promoter: Consitutive lac
• Tag / Fusion Protein:
  • 6 His tag with Thrombin site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: pBBinF (CATCCTGAACTTATCTAGACC)
• 3′ sequencing primer: pBBinR (GCAGGTCCTGAAGTTAACTAG)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry