Skip to main content
Addgene

p3-K230-FokI_R (RR)
(Plasmid #32547)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p3
  • Backbone size w/o insert (bp) 5500
  • Modifications to backbone
    pcDNA3 plasmid with insertion of oligo that contains HA epitope/NLS signal and modified restriction enzyme structure
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZFN
  • Species
    artificial protein
  • Tag / Fusion Protein
    • HA epitope (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fok I nuclease domain of K230 ZFN_R was replaced by an improved version of heterodimer-obligatory nuclease called Sharkey RR (J Mol Biol. 2010 Jul 2;400(1):96-107. Epub 2010 May 4.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3-K230-FokI_R (RR) was a gift from Jin-Soo Kim (Addgene plasmid # 32547 ; http://n2t.net/addgene:32547 ; RRID:Addgene_32547)

  • For your References section:

    Targeted chromosomal deletions in human cells using zinc finger nucleases. Lee HJ, Kim E, Kim JS. Genome Res. 2010 Jan;20(1):81-9. Epub 2009 Dec 1. 10.1101/gr.099747.109 PubMed 19952142
Related items:
Commonly requested with: