p3-K230-FokI_L (wt)
(Plasmid
#32544)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3
- Backbone size w/o insert (bp) 5500
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Modifications to backbonepcDNA3 plasmid with insertion of oligo that contains HA epitope/NLS signal and modified restriction enzyme structure
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZFN
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Alt namezinc finger nucleaes
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Speciesartificial protein
- Promoter CMV
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Tag
/ Fusion Protein
- HA epitope (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3-K230-FokI_L (wt) was a gift from Jin-Soo Kim (Addgene plasmid # 32544 ; http://n2t.net/addgene:32544 ; RRID:Addgene_32544) -
For your References section:
Targeted chromosomal deletions in human cells using zinc finger nucleases. Lee HJ, Kim E, Kim JS. Genome Res. 2010 Jan;20(1):81-9. Epub 2009 Dec 1. 10.1101/gr.099747.109 PubMed 19952142