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pcDNA3-Ha-Brca1 S1423A/S1524A
(Plasmid #32539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Alt name
    BRCC1
  • Alt name
    PNCA4
  • Alt name
    RNF53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5592
  • Mutation
    S1423A/S1524A
  • GenBank ID
    672
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter CMV/rabbit B-Globin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BAMH1 (not destroyed)
  • 3′ cloning site XHO1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA3 HA BRCA1 wild type provided by David Livingston, double mutant made in the Kastan lab at St. Jude Children's Research Hospital

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Ha-Brca1 S1423A/S1524A was a gift from Michael Kastan (Addgene plasmid # 32539 ; http://n2t.net/addgene:32539 ; RRID:Addgene_32539)

  • For your References section:

    Phosphorylation of serine 1387 in Brca1 is specifically required for the Atm-mediated S-phase checkpoint after ionizing irradiation. Xu B, O'Donnell AH, Kim ST, Kastan MB. Cancer Res. 2002 Aug 15;62(16):4588-91. PubMed 12183412
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