pET28-mE1
(Plasmid #32534)

• Depositing Lab: Jorge Eduardo Azevedo
• Publication: Carvalho et al Mol Biotechnol. 2011 Oct 20.

Backbone

• Vector backbone: pET-28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E1, ubiquitin-activating enzyme
• Alt name: UBA1
• Alt name: UBE1
• Species: M. musculus (mouse)
• Insert Size (bp): 3177
• Entrez Gene: Uba1 (a.k.a. RP23-394F6.2, A1S9, AA989744, Sbx, Ube-1, Ube1x)
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original mouse ubiquitin-activating enzyme cDNA was cloned by Dr. Fumiaki Yamao, National Institute of Genetics, Japan.
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry