pcDNA3-IP3KC
(Plasmid
#32527)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/His C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIP3KC
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SpeciesH. sapiens (human)
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Entrez GeneITPKC (a.k.a. IP3-3KC, IP3KC)
- Promoter CMV
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Tag
/ Fusion Protein
- 6xHIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-f
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert contains an internal EcoRI site.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-IP3KC was a gift from Christophe Erneux (Addgene plasmid # 32527 ; http://n2t.net/addgene:32527 ; RRID:Addgene_32527) -
For your References section:
Cloning and expression of a cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase C. Dewaste V, Pouillon V, Moreau C, Shears S, Takazawa K, Erneux C. Biochem J. 2000 Dec 1;352 Pt 2:343-51. 10.1042/0264-6021:3520343 PubMed 11085927