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Addgene

pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
(Plasmid #32520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-CMV-Rheb-IRES-eGFP-W
  • Backbone manufacturer
    Addgene plasmid #32519
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Constitutively active Rheb (S16H)
  • Species
    R. norvegicus (rat)
  • Mutation
    S16H to make constitutively active
  • Entrez Gene
    Rheb
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis.

The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W was a gift from Mustafa Sahin (Addgene plasmid # 32520 ; http://n2t.net/addgene:32520 ; RRID:Addgene_32520)

  • For your References section:

    Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nie D, Di Nardo A, Han JM, Baharanyi H, Kramvis I, Huynh T, Dabora S, Codeluppi S, Pandolfi PP, Pasquale EB, Sahin M. Nat Neurosci. 2010 Feb;13(2):163-72. Epub 2010 Jan 10. 10.1038/nn.2477 PubMed 20062052
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