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Addgene

pOZ-Pax3-FH-C-puro
(Plasmid #32517)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOZ-FH-C-Puro
  • Backbone manufacturer
    Addgene plasmid #32516
  • Backbone size w/o insert (bp) 7900
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)
  • Tag / Fusion Protein
    • FLAG and HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pBMN5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOZ-Pax3-FH-C-puro was a gift from Andrew Lassar (Addgene plasmid # 32517 ; http://n2t.net/addgene:32517 ; RRID:Addgene_32517)

  • For your References section:

    Id3 is a direct transcriptional target of Pax7 in quiescent satellite cells. Kumar D, Shadrach JL, Wagers AJ, Lassar AB. Mol Biol Cell. 2009 Jul;20(14):3170-7. Epub 2009 May 20. 10.1091/mbc.e08-12-1185 PubMed 19458195
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