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pCDNA3.1Xpress-AbrR683AN795A-HisC
(Plasmid #32510)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32510 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABR
  • Alt name
    ABR active BCR-related gene
  • Alt name
    active breakpoint cluster region-related protein isoform b
  • Insert Size (bp)
    2700
  • Mutation
    R 683 to A and N 795 to A
  • GenBank ID
    NM_021962 NP_068781
  • Entrez Gene
    ABR (a.k.a. MDB)
  • Tag / Fusion Protein
    • HISC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note from the depositing lab: An E24G mutation was inadvertently introduced during cloning. There is no evidence that this mutation affects the GAP activity of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1Xpress-AbrR683AN795A-HisC was a gift from John Groffen & Nora Heisterkamp (Addgene plasmid # 32510 ; http://n2t.net/addgene:32510 ; RRID:Addgene_32510)

  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687
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