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Addgene

pSG5-CRKL-Y198FY207F
(Plasmid #32506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRKL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    Y207F and Y198F
  • GenBank ID
    NM_005207
  • Entrez Gene
    CRKL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SMAI (destroyed during cloning)
  • 3′ cloning site SMAI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-CRKL-Y198FY207F was a gift from John Groffen & Nora Heisterkamp (Addgene plasmid # 32506 ; http://n2t.net/addgene:32506 ; RRID:Addgene_32506)

  • For your References section:

    Tyrosine 207 in CRKL is the BCR/ABL phosphorylation site. de Jong R, ten Hoeve J, Heisterkamp N, Groffen J. Oncogene. 1997 Feb 6;14(5):507-13. 10.1038/sj.onc.1200885 PubMed 9053848
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