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Addgene

pCDNA3.1-CIB1-Myc
(Plasmid #32505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIB1
  • Alt name
    CIB1 calcium and integrin binding 1
  • Alt name
    CIB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • GenBank ID
    NM_006384.3
  • Entrez Gene
    CIB1 (a.k.a. CIB, CIBP, KIP1, PRKDCIP, SIP2-28)
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1-CIB1-Myc was a gift from John Groffen & Nora Heisterkamp (Addgene plasmid # 32505 ; http://n2t.net/addgene:32505 ; RRID:Addgene_32505)

  • For your References section:

    The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading. Haataja L, Kaartinen V, Groffen J, Heisterkamp N. J Biol Chem. 2002 Mar 8;277(10):8321-8. Epub 2001 Dec 27. 10.1074/jbc.M105363200 PubMed 11756406
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