pSG5-NRBP-HA
(Plasmid
#32504)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG5
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNRBP
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Alt nameNRBP1 nuclear receptor binding protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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GenBank IDNM_013392.2
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Entrez GeneNRBP1 (a.k.a. BCON3, MADM, MUDPNP, NRBP)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-NRBP-HA was a gift from John Groffen & Nora Heisterkamp (Addgene plasmid # 32504 ; http://n2t.net/addgene:32504 ; RRID:Addgene_32504) -
For your References section:
Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain. De Langhe S, Haataja L, Senadheera D, Groffen J, Heisterkamp N. Int J Mol Med. 2002 May;9(5):451-9. PubMed 11956649