CAG::PSAMQ79G:GlyR-IRES-GFP
(Plasmid #32482)

• Depositing Lab: Scott Sternson
• Publication: Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Backbone

• Vector backbone: pCAGGS-IRES-GFP
• Backbone size w/o insert (bp): 7200
• Modifications to backbone: WPRE (Woodchuck hepatitis virus posttranscriptional regulatory element) before polyA sequence
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSAMQ79G-GlyR
• Species: H. sapiens (human)
• Insert Size (bp): 1295
• Promoter: CAG
• Tag / Fusion Protein:
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CTGCTAACCATGTTCATGC
• 3′ sequencing primer: GAAGACTCTTGCGTTTCTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry