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Addgene

CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
(Plasmid #32474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS- IRES-GFP
  • Backbone size w/o insert (bp) 7200
  • Modifications to backbone
    WPRE (Woodchuck hepatitis virus posttranscriptional regulatory element) before polyA sequence
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSAMQ79G,Q139G:5HT3 HC
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    1415
  • Promoter CAG
  • Tag / Fusion Protein
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer CTGCTAACCATGTTCATGC
  • 3′ sequencing primer CAGAAACGCAAGAGTCTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP was a gift from Scott Sternson (Addgene plasmid # 32474 ; http://n2t.net/addgene:32474 ; RRID:Addgene_32474)

  • For your References section:

    Chemical and genetic engineering of selective ion channel-ligand interactions. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM. Science. 2011 Sep 2;333(6047):1292-6. 10.1126/science.1206606 PubMed 21885782
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