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Addgene

Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D
(Plasmid #32460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLsin.PPT.hPGK.FLAG-CaMKKbeta-IRES.GFP
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase beta
  • Alt name
    CaMKKbeta
  • Alt name
    CaMKK2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600
  • Mutation
    S129D, S133D, S137D
  • GenBank ID
    NM_145358.1
  • Entrez Gene
    Camkk2 (a.k.a. 6330570N16Rik, AW061083, mKIAA0787)
  • Promoter human PGK
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Insert specific
  • 3′ sequencing primer Insert specific
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D was a gift from Anthony Means (Addgene plasmid # 32460 ; http://n2t.net/addgene:32460 ; RRID:Addgene_32460)

  • For your References section:

    Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR. J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. 10.1074/jbc.M111.251504 PubMed 21669867
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