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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D
(Plasmid #32457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4100
  • Modifications to backbone
    Added blunt end cloning site which was lost during cloning.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase beta
  • Alt name
    CaMKKbeta
  • Alt name
    CaMKK2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1700
  • Mutation
    S129D, S133D, S137D (S3V, G96R, and P108L also present)
  • GenBank ID
    NM_031338
  • Entrez Gene
    Camkk2
  • Promoter SV40
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (destroyed during cloning)
  • 3′ cloning site None (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D was a gift from Anthony Means (Addgene plasmid # 32457 ; http://n2t.net/addgene:32457 ; RRID:Addgene_32457)

  • For your References section:

    Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR. J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. 10.1074/jbc.M111.251504 PubMed 21669867
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