pEGFP-N1-CaMKKbeta S129D, S133D, S137D
(Plasmid
#32455)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Modifications to backboneFLAG tag added into NheI site by cutting and inserting linker with FLAG tag and NheI overhangs. Second NheI site removed by site-directed mutagenesis.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCalcium/Calmodulin dependent protein kinase kinase beta
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Alt nameCaMKKbeta
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Alt nameCaMKK2
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SpeciesM. musculus (mouse)
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MutationS129D, S133D, S137D
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GenBank IDNM_145358.1
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Entrez GeneCamkk2 (a.k.a. 6330570N16Rik, AW061083, mKIAA0787)
- Promoter CMV
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Tags
/ Fusion Proteins
- GFP (C terminal on backbone)
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
W374C mutation present.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-CaMKKbeta S129D, S133D, S137D was a gift from Anthony Means (Addgene plasmid # 32455 ; http://n2t.net/addgene:32455 ; RRID:Addgene_32455) -
For your References section:
Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR. J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. 10.1074/jbc.M111.251504 PubMed 21669867