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pSG5-FLAG-CaMKKbeta rat FL
(Plasmid #32449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4100
  • Modifications to backbone
    Added blunt end cloning site which was lost during cloning.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase beta
  • Alt name
    CaMKKbeta
  • Alt name
    CaMKK2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1700
  • Mutation
    S3L, G96R, and P108L
  • GenBank ID
    NM_031338
  • Entrez Gene
    Camkk2
  • Promoter SV40
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (destroyed during cloning)
  • 3′ cloning site None (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The S3L, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-FLAG-CaMKKbeta rat FL was a gift from Anthony Means (Addgene plasmid # 32449 ; http://n2t.net/addgene:32449 ; RRID:Addgene_32449)

  • For your References section:

    Hypothalamic CaMKK2 contributes to the regulation of energy balance. Anderson KA, Ribar TJ, Lin F, Noeldner PK, Green MF, Muehlbauer MJ, Witters LA, Kemp BE, Means AR. Cell Metab. 2008 May;7(5):377-88. 10.1016/j.cmet.2008.02.011 PubMed 18460329
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