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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGluR1
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SpeciesH. sapiens (human)
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MutationContains the last C-terminal 81 amino acids of the GluR1 subunit
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Entrez GeneGRIA1 (a.k.a. GLUH1, GLUR1, GLURA, GluA1, HBGR1, MRD67, MRT76)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40par (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC1-GluR1Ctail was a gift from Robert Malinow (Addgene plasmid # 32441 ; http://n2t.net/addgene:32441 ; RRID:Addgene_32441) -
For your References section:
Postsynaptic receptor trafficking underlying a form of associative learning. Rumpel S, LeDoux J, Zador A, Malinow R. Science. 2005 Apr 1;308(5718):83-8. Epub 2005 Mar 3. 10.1126/science.1103944 PubMed 15746389