LHP1032 - EYFP-Nterm
(Plasmid
#32438)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1 TOPO
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Backbone manufacturerInvitrogen
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For the addition of EYFP to the N-terminus of another cDNA by cloning the cDNA into the NotI site (of the original pCR2.1 TOPO).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LHP1032 - EYFP-Nterm was a gift from Linda Hicke (Addgene plasmid # 32438 ; http://n2t.net/addgene:32438 ; RRID:Addgene_32438)