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Addgene

LHP1463 - INP52
(Plasmid #32432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac22
  • Backbone manufacturer
    ATCC
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INP52
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    INP52 (a.k.a. YNL106C, SJL2)
  • Promoter Lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

INP52 was amplified from yeast genomic DNA using 5′ PstI and 3′ BamHI site-containing oligonucleotides, digested and ligated into PstI- and BamHI-digested YCplac22. The NotI site was inserted at the 5′-end of INP52 via QuikChange™ mutagenesis. A triple HA epitope was ligated into the NotI site to generate pHA-INP52 (LHP1463).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LHP1463 - INP52 was a gift from Linda Hicke (Addgene plasmid # 32432 ; http://n2t.net/addgene:32432 ; RRID:Addgene_32432)

  • For your References section:

    The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. Stamenova SD, Dunn R, Adler AS, Hicke L. J Biol Chem. 2004 Apr 16;279(16):16017-25. Epub 2004 Feb 3. 10.1074/jbc.M313479200 PubMed 14761940
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