LHP1263 - HA-VPS9
(Plasmid #32431)

• Depositing Lab: Linda Hicke
• Publication: Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

Backbone

• Vector backbone: YCplac111
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VPS9
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: VPS9 (a.k.a. YML097C, VPL31, VPT9)
• Promoter: Lac
• Tag / Fusion Protein:
  • 3xHA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: M13 reverse
• 3′ sequencing primer: M13 forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To insert an HA epitope at the N-terminus of Vps9, a NotI site was introduced after the first methionine codon of VPS9 in pPS91 (Hama et al., 1999). NotI-VPS9 was then subcloned into the centromere-based vector YCplac111 and a fragment encoding an 3xHA tag flanked by NotI sites was inserted.