pENTR-L3-oPRE-L2
(Plasmid #32415)

• Depositing Lab: Matthew Nolan
• Publication: White et al Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.

Backbone

• Vector backbone: pDONR221 P3-P2
• Backbone manufacturer: Invitrogen
• Vector type: Gateway cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: oPRE
• Alt name: optimised version of WPRE
• Mutation: WPRE with deletion of X protein promoter and mutation of all four ATGs which are followed by an ORF encoding more than 25 amino acids

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMP71 GFP oPRE Provided by Dr Axel Schambach the user of the oPRE should cite the original article describing the element by Axel Schambach of Hannover Biomedical Research School

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005

'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.'

Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs